HCC xenograft tumour tissues
SMMC7721; Huh7; LO2
cell line-derived xenograft
migration (promotes); apoptosis (inhibits); ceRNA regulation (other); cell cycle (other); p53 signaling (other)
RNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; In Vivo Animal Model
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hsa_circ_0088364 is upregulated in HCC cells versus normal hepatocytes and functionally promotes HCC cell migration while inhibiting NC-induced apoptosis. It was validated as differentially expressed in NC-treated vs untreated HCC xenograft tumors and is predicted to participate in a circRNA-miRNA-mRNA (ceRNA-like) regulatory network.
0.6111
High throughput circRNA sequencing analysis reveals novel insights into the mechanism of nitidine chloride against hepatocellular carcinoma.
combined biological and clinical study