| Expression pattern: |
UP |
| Associated gene: |
CASR |
| Associated microRNA: |
miR-185-3p |
| Biological function: |
Promotes cardiac hypertrophy; circHIPK3 silencing attenuates TAC- and Ang II-induced cardiomyocyte hypertrophy and cardiac dysfunction. |
| Molecular mechanism: |
Acts as a miR-185-3p sponge to modulate CASR (ceRNA mechanism). |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for treating cardiac hypertrophy. |
| Description: |
circHIPK3 is up-regulated in TAC- and Ang II-induced cardiac hypertrophy and mainly localizes to the cytoplasm. circHIPK3 promotes hypertrophic phenotypes by sponging miR-185-3p, thereby relieving miR-185-3p-mediated repression of CASR; circHIPK3 knockdown attenuates hypertrophy and dysfunction in vitro and in vivo. |
| Confidence score: |
0.8152 |