circRNA basic information
circBase ID: hsa_circ_0000977
Name: hsa_circ_NOL10
Synonym: CircNOL10
Host Gene: NOL10
Genomic location(hg19): chr2:10784445-10808849:-
Genomic location(hg38): chr2:10644319-10668723:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; adjacent non-cancerous specimens
Cell lines:

MCF-7; MDA-MB-231; MDA-MB-468; SKBR-3; BT-549; BT-474; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: SOCS2, Ago2, JAK2, STAT5
Associated microRNA: miR-767-5p
Biological function: inhibits cell proliferation, migration, invasion and EMT; suppresses tumor growth in vivo
Molecular mechanism: Acts as a sponge for miR-767-5p to up-regulate SOCS2 and inactivate JAK2/STAT5 signaling.
Biological pathway or process:

JAK/STAT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); metastasis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low expression of circNOL10 was associated with larger tumor size, advanced TNM stage, lymph node metastasis and unfavorable prognosis.
Description:

In breast cancer, circNOL10 is down-regulated and functions as a tumor suppressor. It mainly localizes to the cytoplasm and sponges miR-767-5p, thereby de-repressing SOCS2 and inhibiting JAK2/STAT5 signaling, which reduces proliferation, migration, invasion, EMT, and xenograft tumor growth.
Confidence score:

0.8912
Other information
Title:

CircNOL10 suppresses breast cancer progression by sponging miR-767-5p to regulate SOCS2/JAK/STAT signaling.

Journal: Journal of biomedical science
Published: 2021
PubMed ID: 33397365
Study type:

combined biological and clinical study
Data availability: GSE101123
Code availability: -