| Expression pattern: |
DN |
| Associated gene: |
HuR, MMP1 mRNAs, KIAA1429, DHX9 |
| Associated microRNA: |
- |
| Biological function: |
inhibits proliferation; inhibits migration and invasion; suppresses tumor growth and metastasis |
| Molecular mechanism: |
circDLC1 binds the RNA-binding protein HuR and reduces HuR-MMP1 mRNA interaction, thereby reducing MMP1 mRNA stability and expression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); cell cycle (inhibits); mRNA stability (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; Northern Blot |
| Clinical significance: |
low circDLC1 expression predicted poor prognosis in HCC patients; circDLC1 is a promising prognostic marker for HCC patients |
| Description: |
circDLC1 (hsa_circ_0135718), derived from the DLC1 gene, is down-regulated in HCC tissues and its low expression predicts worse OS/RFS. Functionally, circDLC1 suppresses hepatoma cell proliferation, cell-cycle progression, migration/invasion and in vivo tumor growth/metastasis by binding HuR and weakening HuR-MMP1 mRNA interaction, which reduces MMP1 mRNA stability and expression. |
| Confidence score: |
0.8897 |