| Expression pattern: |
UP |
| Associated gene: |
Rap1b, VEGFR2, PI3K, AKT, VEGF-A, CD31 |
| Associated microRNA: |
miR-30c-5p |
| Biological function: |
Promotes LC progression, proliferation, migration, invasion, tumor growth and angiogenesis; inhibits apoptosis; exosomal circPVT1 enhances endothelial cell migration and tube formation. |
| Molecular mechanism: |
Exosomal circPVT1 acts as a miR-30c-5p sponge to upregulate Rap1b, activating VEGFR2 and PI3K/AKT signaling to promote angiogenesis. |
| Biological pathway or process: |
VEGF/VEGFR (promotes); PI3K/AKT (promotes); angiogenesis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); drug resistance (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; IHC (Immunohistochemistry); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; Western Blot; Luciferase Reporter Assay; ELISA; Bioinformatics Analysis; Survival Analysis; Cohort Study |
| Clinical significance: |
High circPVT1 expression is correlated with advanced T stage and lower survival rate; exosomal circPVT1 may serve as a valuable biomarker, prognostic marker and therapeutic target for LC. |
| Description: |
circPVT1 is upregulated in laryngeal cancer tissues, cell lines and patient plasma/serum exosomes, and high expression is associated with disease progression and poorer survival. LC cell-derived exosomal circPVT1 is transferred to endothelial cells, where it sponges miR-30c-5p to increase Rap1b and activate VEGFR2/PI3K/AKT signaling, promoting angiogenesis. Targeting circPVT1 suppresses tumor growth and angiogenesis and may improve the therapeutic effect of cetuximab. |
| Confidence score: |
0.8558 |