| Expression pattern: |
UP |
| Associated gene: |
LASP1 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Facilitates cell growth/proliferation and limits apoptosis in colorectal cancer cells. |
| Molecular mechanism: |
Acts as a miRNA sponge regulating the miR-326/LASP1 axis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Higher expression of hsa_circRNA_000166 was associated with poor 5-year survival rate of patients with CRC. |
| Description: |
hsa_circRNA_000166 is upregulated in colorectal cancer tissues and cell lines and its high expression is associated with worse 5-year survival. Functionally, it promotes CRC cell growth and inhibits apoptosis by sponging miR-326, thereby regulating the downstream effector LASP1. |
| Confidence score: |
0.7357 |