circRNA basic information
circBase ID: -
Name: hsa_circ_ASPH
Synonym: circASPH
Host Gene: ASPH
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0008487
MONDO name: polycystic ovary syndrome
Disease details: Polycystic Ovary Syndrome
Disease DO ID:
11612
Disease MeSH ID:
D011085
Disease NCIt ID:
C26862
Disease ICD11 ID:
1213633323
Disease OMIM ID:
184700
Species: Human
Species details: Homo sapiens
Tissue specimen:

ovarian granulosa cells

Cell lines:

KGN; SVOG

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: MAP2K6
Associated microRNA: miR-375
Biological function: promotes KGN cells proliferation; inhibits apoptosis
Molecular mechanism: circASPH directly targets (sponges) miR-375 and positively regulates MAP2K6 via a ceRNA network
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; Bioinformatics Analysis

Clinical significance:

thought-provoking biomarkers for PCOS diagnosis and therapy

Description:

circASPH is up-regulated in PCOS granulosa cells and promotes granulosa-like (KGN) cell proliferation while inhibiting apoptosis. Mechanistically, circASPH sponges miR-375 to relieve repression of MAP2K6, forming a circASPH/miR-375/MAP2K6 ceRNA axis with potential diagnostic/therapeutic relevance in PCOS.

Confidence score:

0.6521

Other information
Title:

CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in Polycystic Ovary Syndrome.

Journal: Journal of cellular and molecular medicine
Published: 2022
PubMed ID: 33372369
Study type:

combined biological and clinical study

Data availability: The data used to support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -