| Expression pattern: |
UP |
| Associated gene: |
TRIM23, Ago2 |
| Associated microRNA: |
miR-377 |
| Biological function: |
Promotes proliferation and glycolysis and inhibits apoptosis; promotes tumor growth in vivo in hepatocellular carcinoma. |
| Molecular mechanism: |
circPVT1 acts as a ceRNA by sponging miR-377 to upregulate TRIM23; interaction supported by dual-luciferase reporter assay and Ago2-RIP. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
CircPVT1/miR-377/TRIM23 can act as novel biomarkers and clinical targets for HCC. |
| Description: |
circPVT1 is up-regulated in hepatocellular carcinoma tissues and cell lines, and functions as an oncogenic circRNA. It primarily localizes in the cytoplasm and promotes proliferation and glycolysis while inhibiting apoptosis by sponging miR-377 to increase TRIM23 expression, thereby enhancing tumor growth in xenograft models. |
| Confidence score: |
0.762 |