| Expression pattern: |
UP |
| Associated gene: |
REEP3, AGO2 |
| Associated microRNA: |
miR-30a-5p |
| Biological function: |
promotes proliferation, invasion, EMT and tumorigenesis in hepatocellular carcinoma |
| Molecular mechanism: |
circFAT1 acts as a miRNA sponge for miR-30a-5p to upregulate REEP3 (ceRNA mechanism) |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; ELISA; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circFAT1 level is positively correlated with TNM stage and tumour size; potential diagnostic and therapeutic biomarker/target in HCC |
| Description: |
circFAT1 is up-regulated in hepatocellular carcinoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes HCC proliferation, invasion, EMT and xenograft tumor growth by acting as a ceRNA that sponges miR-30a-5p, thereby increasing REEP3 expression. |
| Confidence score: |
0.7631 |