| Expression pattern: |
UP |
| Associated gene: |
FCHSD2 |
| Associated microRNA: |
- |
| Biological function: |
Modulates chemoresistance; knockdown increases viability under drug treatment (reduced sensitivity) and authors propose dual roles (tumor-suppressive in cells vs oncogenic in exosomes). |
| Molecular mechanism: |
Not experimentally resolved; bioinformatics prediction of MREs/targets for upregulated circRNAs; functional effect tested by siRNA knockdown. |
| Biological pathway or process: |
drug resistance (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Cell Cycle Assay |
| Clinical significance: |
Differentially upregulated hsa_circ_0000338 in exosomes could serve as a potential biomarker for early prediction of chemoresistance in CRC. |
| Description: |
hsa_circ_0000338 (circ_0000338), derived from FCHSD2, is upregulated in exosomes from FOLFOX-resistant HCT116-R CRC cells. siRNA knockdown in resistant cells increased drug-treated viability, and the authors propose a dual role with tumor-suppressive properties in cells but oncogenic effects when transferred via exosomes; it is suggested as a potential biomarker of chemoresistance. |
| Confidence score: |
0.7399 |