| Expression pattern: |
UP |
| Associated gene: |
LARP1, mTOR |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
promotes cell viability, proliferation, migration and invasion; promotes tumor growth and metastasis; inhibits apoptosis; promotes EMT |
| Molecular mechanism: |
ceRNA (miRNA sponge) mechanism: hsa_circ_002144 binds miR-615-5p, reduces miR-615-5p, leading to increased LARP1 and mTOR expression to promote CRC progression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); mTOR (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); RNA-seq |
| Clinical significance: |
High hsa_circ_002144 expression was closely associated with shorter overall survival and metastatic clinicopathological features in CRC patients; not an independent prognostic biomarker by multivariate Cox regression. |
| Description: |
hsa_circ_002144 is up-regulated in colorectal cancer tissues and cell lines and is associated with shorter overall survival. Functionally, it promotes CRC growth, metastasis and EMT while inhibiting apoptosis, mainly by sponging miR-615-5p to increase LARP1 and mTOR expression. |
| Confidence score: |
0.8192 |