| Expression pattern: |
UP |
| Associated gene: |
YTHDF3, Wnt3, Wnt3 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes glioma cell migration, invasion, EMT, tumorigenicity, and lung metastasis. |
| Molecular mechanism: |
circ_EPHB4 promotes glioma progression by upregulating Wnt3 expression and synergizing with the m6A reader YTHDF3, which stabilizes Wnt3 mRNA in an m6A-dependent manner. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); mRNA stability (promotes); m6A modification (other); other pathway/process (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RT-qPCR; RNA-seq; Microarray; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Bioinformatics Analysis |
| Clinical significance: |
circ_EPHB4 is suggested as a valuable prognostic marker and a potential therapeutic target in glioma. |
| Description: |
circ_EPHB4 is up-regulated in glioma tissues and glioma cell lines. It promotes glioma migration, invasion, EMT, tumor growth, and lung metastasis, at least partly by enhancing Wnt3 expression and cooperating with the m6A reader YTHDF3 to regulate Wnt3 mRNA stability. The study suggests the circ_EPHB4-YTHDF3-Wnt3 axis as a potential therapeutic target in glioma. |
| Confidence score: |
0.8244 |