| Expression pattern: |
UP |
| Associated gene: |
MECP2, Ago2, ASH2L |
| Associated microRNA: |
miR-140-3p |
| Biological function: |
Circ_0136474 promotes osteoarthritis progression and IL-1beta-induced chondrocyte injury; circ_0136474 knockdown facilitates cell proliferation and restrains apoptosis, ECM degradation, and inflammatory response. |
| Molecular mechanism: |
ceRNA mechanism: circ_0136474 acts as a sponge for miR-140-3p and positively regulates MECP2 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
provided a possible target for gene therapy |
| Description: |
Circ_0136474 is up-regulated in osteoarthritis cartilage tissues and IL-1beta-treated CHON-001 cells. It promotes OA progression by sponging miR-140-3p and upregulating MECP2, thereby enhancing chondrocyte apoptosis, ECM degradation, and inflammation while suppressing cell proliferation. |
| Confidence score: |
0.7785 |