| Expression pattern: |
UN |
| Associated gene: |
SLC2A1 |
| Associated microRNA: |
miR-320-5p |
| Biological function: |
promotes proliferation and migration; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circHECTD1 sponges miR-320-5p to upregulate SLC2A1. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; Cell Cycle Assay; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circHECTD1 promotes glioblastoma multiform cell proliferation and migration and enhances tumor growth in xenografts. It functions as a ceRNA that sponges miR-320-5p, thereby increasing SLC2A1 expression. |
| Confidence score: |
0.8143 |