| Expression pattern: |
UP |
| Associated gene: |
ZNF281, AGO2 |
| Associated microRNA: |
miR‐488 |
| Biological function: |
promotes NSCLC cell proliferation and invasion and promotes tumor growth in vivo |
| Molecular mechanism: |
circ‐0008003 acts as a ceRNA by sponging miR‐488 to de-repress ZNF281 in NSCLC |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circ‐0008003 level was associated with TNM stage and lymphatic metastasis, and NSCLC patients with high circ‐0008003 level had poor prognosis |
| Description: |
hsa_circ_0008003 (circ-0008003) is up-regulated in NSCLC tissues and cell lines and promotes proliferation, invasion, and xenograft tumor growth. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-488 to increase ZNF281 expression. High circ-0008003 level is associated with advanced TNM stage/lymphatic metastasis and predicts poor survival. |
| Confidence score: |
0.8581 |