| Expression pattern: |
UP |
| Associated gene: |
PIK3R3, AKT, mTOR, AGO2 |
| Associated microRNA: |
miR-193a-5p |
| Biological function: |
promotes proliferation and invasion; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-193a-5p to regulate PIK3R3, promoting AKT/mTOR pathway activation |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); PI3K/AKT/mTOR (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circRNA_0000392 could discriminate CRC from adjacent normal tissues (AUC 0.713) and its expression was correlated with pathological stage, lymph node metastasis and distant metastasis |
| Description: |
circRNA_0000392 is up-regulated in colorectal cancer and promotes malignant phenotypes (proliferation, invasion, tumor growth). It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-193a-5p, thereby increasing PIK3R3 and activating the AKT/mTOR pathway. |
| Confidence score: |
0.8768 |