| Expression pattern: |
UP |
| Associated gene: |
PGK1, Ago2 |
| Associated microRNA: |
miR-708-5p |
| Biological function: |
promotes proliferation; promotes glycolysis; enhances paclitaxel (PTX) IC50; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circ_0001955 functions as a ceRNA sponge for miR-708-5p, thereby upregulating PGK1 and promoting glycolysis and malignant phenotypes in breast cancer; circ_0001955 is packaged into exosomes and transferred between cells. |
| Biological pathway or process: |
proliferation (promotes); glycolysis (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; EdU; Colony Formation Assay; MTT; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High circ_0001955 level is associated with poorer overall survival in breast cancer patients. |
| Description: |
circ_0001955 is up-regulated in breast cancer tissues/cells and is enriched in exosomes. It promotes proliferation and tumor growth, enhances paclitaxel resistance (higher IC50), and stimulates glycolysis by sponging miR-708-5p to upregulate PGK1. |
| Confidence score: |
0.8571 |