| Expression pattern: |
UP |
| Associated gene: |
FKBP10, CHD7, RIG-1, RNF125 |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC tumorigenesis, proliferation, invasion/metastasis and stem cell-like phenotype; restricts antitumor immunity |
| Molecular mechanism: |
circREEP3 recruits CHD7 to FKBP10 promoter to activate FKBP10 transcription; promotes RNF125-dependent ubiquitination and proteasomal degradation of RIG-1, suppressing RIG-1 signaling and antitumor immunity |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); immune regulation (inhibits); other pathway/process (other) |
| Detected method: |
Q
B
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Northern Blot; FISH / smFISH; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; Co-IP; ELISA; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
circREEP3 upregulation predicts poor patient survival; circREEP3 level was increased in CRC tissues and positively correlated with clinical severity, metastasis and prognosis |
| Description: |
circREEP3 is up-regulated in colorectal cancer and its high expression predicts poor survival. Functionally, it promotes CRC proliferation, invasion/metastasis and stemness. Mechanistically, circREEP3 activates FKBP10 transcription by recruiting CHD7 to the FKBP10 promoter and suppresses RIG-1-dependent antitumor immunity by enhancing RNF125-mediated RIG-1 degradation. |
| Confidence score: |
0.8998 |