circRNA basic information
circBase ID: hsa_circ_0002874
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr9:4286037-4286523:-
Genomic location(hg38): chr9:4286037-4286523:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; paired non-cancerous matched tissues; tumor tissues

Cell lines:

A549; A549/Taxol

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MDM2, P53
Associated microRNA: hsa-miR-1273f
Biological function: promotes paclitaxel resistance and affects apoptosis in NSCLC
Molecular mechanism: ceRNA mechanism: hsa_circ_0002874 sponges miR1273f to regulate MDM2 and P53
Biological pathway or process:

apoptosis (inhibits); chemoresistance (promotes); p53 signaling (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; MTT; CCK8; Colony Formation Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis

Clinical significance:

strong expression of hsa_circ_0002874 in NSCLC tissues; increased expression related to advanced TNM stage; potential marker of PTX resistance

Description:

hsa_circ_0002874 is upregulated in NSCLC tissues and is associated with paclitaxel resistance. Mechanistically, it functions as a ceRNA by sponging miR-1273f to modulate the MDM2/P53 signaling, thereby affecting apoptosis and chemosensitivity; knockdown of hsa_circ_0002874 (or miR-1273f overexpression) reverses PTX resistance in vitro and in xenograft models.

Confidence score:

0.7126

Other information
Title:

Overexpression of hsa_circ_0002874 promotes resistance of non-small cell lung cancer to paclitaxel by modulating miR-1273f/MDM2/p53 pathway.

Journal: Aging
Published: 2021
PubMed ID: 33612481
Study type:

combined biological and clinical study

Data availability: -
Code availability: -