circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: -
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent tissues; xenograft tumors

Cell lines:

Huh7; MHCC-LM3; HepG2; SMMC-7721; PLC; L02; HEK293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: AQP3
Associated microRNA: miR-124
Biological function: promotes proliferation and migration; promotes xenograft tumor growth
Molecular mechanism: acts as a miR-124 sponge to regulate the miR-124 target gene AQP3
Biological pathway or process:

proliferation (promotes); migration (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Sanger Sequencing; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation

Clinical significance:

high expression level of circHIPK3 was correlated with tumor differentiation; TNM stage; HBV-DNA copy numbers; and the presence of liver cirrhosis

Description:

circHIPK3 is up-regulated in HCC tissues and promotes HCC cell proliferation and migration. Mechanistically, circHIPK3 sponges miR-124 to de-repress the miR-124 target AQP3, and circHIPK3 knockdown suppresses Huh7 xenograft tumor growth via the miR-124-AQP3 axis.

Confidence score:

0.7471

Other information
Title:

circHIPK3 regulates cell proliferation and migration by sponging miR-124 and regulating AQP3 expression in hepatocellular carcinoma.

Journal: Cell death & disease
Published: 2018
PubMed ID: 29415990
Study type:

combined biological and clinical study

Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-017-0204-3).
Code availability: -