circRNA basic information
circBase ID: hsa_circ_0001445
Name: hsa_circ_SMARCA5
Synonym: circSMARCA5
Host Gene: SMARCA5
Genomic location(hg19): chr4:144464661-144465125:+
Genomic location(hg38): chr4:143543508-143543972:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBM biopsies; normal brain parenchyma
Cell lines:

U87MG; A172; CAS1; DBTRG; SNB19
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: SRSF1, SRSF3, PTBP1, PTBP2
Associated microRNA: -
Biological function: inhibits migration
Molecular mechanism: Acts as an RBP modulator by tethering SRSF1 and affecting SRSF3 splicing (exon 4 inclusion), with downstream links to PTBP1/PTBP2.
Biological pathway or process:

migration (inhibits); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; Northern Blot; Transfection; Clinical Sample Validation; Bioinformatics Analysis; MTT; In Vivo Animal Model; RIP (RNA Immunoprecipitation)
Clinical significance:

circSMARCA5 expression negatively correlates with glioma histological grade; ROC curves suggest circSMARCA5 as a candidate GBM biomarker.
Description:

circSMARCA5 (hsa_circ_0001445) is significantly down-regulated in GBM patient biopsies and GBM cell lines. Its overexpression suppresses GBM cell migration. Mechanistically, it likely functions as an RBP modulator by tethering SRSF1, altering SRSF3 splicing (exon 4 inclusion) and linking to a splicing-factor network involving PTBP1/PTBP2.
Confidence score:

0.6676
Other information
Title:

CircSMARCA5 Inhibits Migration of Glioblastoma Multiforme Cells by Regulating a Molecular Axis Involving Splicing Factors SRSF1/SRSF3/PTB.

Journal: International journal of molecular sciences
Published: 2018
PubMed ID: 29415469
Study type:

combined biological and clinical study
Data availability: TCGA GBM RNA-seq data (Cancer RNA-Seq Nexus database); ENCODE eCLIP bam accessions ENCFF913MGS; ENCFF374XWQ; ENCFF833PXB
Code availability: -