| Expression pattern: |
UP |
| Associated gene: |
METTL3, YTHDF1, FOXM1, PCNA, p21 |
| Associated microRNA: |
miR-761, miR-4778-3p |
| Biological function: |
circRPS6KC1 promotes proliferation and cell cycle progression, inhibits cellular senescence, and supports tumor growth in prostate cancer; suppression of circRPS6KC1 induces G1/G0 arrest, decreases DNA replication, increases SA-beta-gal-positive senescent cells, and reduces xenograft tumor growth. |
| Molecular mechanism: |
METTL3 and YTHDF1 mediate m6A modification and stabilization of circRPS6KC1. Cytoplasmic circRPS6KC1 functions as a miR-761 sponge to regulate FOXM1 expression; FOXM1 transcriptionally regulates PCNA and influences p21 degradation in a p53-independent manner. |
| Biological pathway or process: |
m6A modification (other); ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); other pathway/process (inhibits); ubiquitination (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; circRNA-seq; RNA-seq; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circRPS6KC1 is an up-regulated human circRNA in prostate cancer derived from RPS6KC1. METTL3/YTHDF1-mediated m6A modification stabilizes circRPS6KC1, and circRPS6KC1 promotes proliferation and suppresses cellular senescence by sponging miR-761 to regulate FOXM1. FOXM1 then regulates PCNA transcription and p21 protein degradation in a p53-independent pathway. |
| Confidence score: |
0.8872 |