circRNA basic information
circBase ID: hsa_circ_0001459
Name: hsa_circ_NEIL3
Synonym: circular RNA nei-like DNA glycosylase 3
Host Gene: NEIL3
Genomic location(hg19): chr4:178274461-178274882:+
Genomic location(hg38): chr4:177353307-177353728:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues
Cell lines:

HuH-7; HCCLM3; Hep3B; MHCC97L; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LAMC1, Ago2 protein
Associated microRNA: miR-3150b-3p
Biological function: circNEIL3 acts as an oncogenic circRNA in HCC; its knockdown suppresses HCC cell proliferation, migration, invasion, and tumor growth, while inducing apoptosis and G0/G1 cell cycle arrest.
Molecular mechanism: circNEIL3 functions as a ceRNA/miRNA sponge for miR-3150b-3p and indirectly upregulates LAMC1, thereby promoting HCC progression through the miR-3150b-3p/LAMC1 regulatory network.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

High circNEIL3 expression was significantly associated with tumor size, TNM stage, and lymph node metastases in HCC patients.
Description:

circNEIL3 (hsa_circ_0001459), derived from NEIL3, is up-regulated in HCC tumor tissues and cell lines and mainly localizes to the cytoplasm. It promotes HCC cell proliferation, migration, invasion, cell-cycle progression, and in vivo tumor growth while suppressing apoptosis. Mechanistically, circNEIL3 functions as a ceRNA for miR-3150b-3p to indirectly increase LAMC1 expression, forming a circNEIL3/miR-3150b-3p/LAMC1 regulatory network.
Confidence score:

0.8175
Other information
Title:

Circular ribonucleic acid nei-like deoxyribonucleic acid glycosylase 3 governs the microribonucleic acid -3150b-3p/laminin subunit gamma 1 network to partially promote the development of hepatocellular carcinoma.

Journal: Hepatology research : the official journal of the Japan Society of Hepatology
Published: 2021
PubMed ID: 33738908
Study type:

combined biological and clinical study
Data availability: starBase (http://starbase.sysu.edu.cn/); GEPIA (http://gepia.cancer-pku.cn/)
Code availability: -