| Expression pattern: |
UP |
| Associated gene: |
HDGF |
| Associated microRNA: |
miR-654-3p |
| Biological function: |
promotes AML cell proliferation/growth and inhibits apoptosis |
| Molecular mechanism: |
ceRNA sponge of miR-654-3p leading to increased HDGF expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; FISH / smFISH; RNase R Treatment; Actinomycin D / DRB Stability Assay; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation |
| Clinical significance: |
may function as a new treatment target for AML therapy |
| Description: |
hsa_circ_0079480 is up-regulated in AML bone marrow and mainly localized in the cytoplasm. It promotes AML cell proliferation and suppresses apoptosis by acting as a ceRNA that sponges miR-654-3p, thereby relieving inhibition of HDGF; rescue experiments support the hsa_circ_0079480/miR-654-3p/HDGF axis as the mechanism. |
| Confidence score: |
0.8267 |