| Expression pattern: |
DN |
| Associated gene: |
RTN4 |
| Associated microRNA: |
miR-338-5p |
| Biological function: |
inhibits HCC cell proliferation and promotes apoptosis (in vitro and in vivo) |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0032683 sponges miR-338-5p to up-regulate RTN4 |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; Western Blot; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
Low hsa_circ_0032683 expression was associated with tumor size and tumor number; patients with low expression had worse survival (high expression associated with better prognosis). |
| Description: |
In hepatocellular carcinoma, hsa_circ_0032683 is down-regulated in tumors and HCC cell lines. It suppresses proliferation and promotes apoptosis by acting as a cytoplasmic ceRNA that sponges miR-338-5p, thereby increasing RTN4 expression; higher circRNA levels are associated with better patient survival. |
| Confidence score: |
0.8156 |