| Expression pattern: |
DN |
| Associated gene: |
IRF2, Ago2 |
| Associated microRNA: |
miR-1227 |
| Biological function: |
Inhibits osteosarcoma cell proliferation, colony formation, invasion and migration; promotes apoptosis; restricts G1-to-S transition; suppresses tumour growth and lung metastasis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-1227 to relieve repression of IRF2; circ-0000658 and miR-1227 are enriched in Ago2-RIP and bind as shown by dual-luciferase reporter assays; functional rescue supports the miR-1227/IRF2 axis. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); apoptosis (promotes); cell cycle (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; TUNEL; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-0000658 expression is associated with longer overall survival; low expression correlates with distant metastasis, tumour size and clinical stage. |
| Description: |
hsa_circ_0000658 is down-regulated in osteosarcoma tissues/cell lines and acts as a tumor suppressor. It sponges miR-1227 to increase IRF2, thereby inhibiting proliferation/invasion, promoting apoptosis, and suppressing tumour growth and lung metastasis; higher circ-0000658 associates with better overall survival. |
| Confidence score: |
0.8428 |