circRNA basic information
circBase ID: hsa_circ_0000594
Name: hsa_circ_TTBK2
Synonym: -
Host Gene: TTBK2
Genomic location(hg19): chr15:43120125-43164956:-
Genomic location(hg38): chr15:42827927-42872758:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; matched normal adjacent tissue samples; tumor tissues; adjacent tissues
Cell lines:

H1755; H1299; A549; MES-1; H226; H661; BEAS-2B; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TEAD1, DERL1, Ago2
Associated microRNA: miR-873-5p
Biological function: circTTBK2 acts as an oncogenic circRNA in NSCLC, promoting cell proliferation, migration, invasion and tumor growth, and its overexpression is associated with TNM stage, lymph node metastasis and poorer survival.
Molecular mechanism: circTTBK2 functions as a cytoplasmic miRNA sponge for miR-873-5p in a RISC manner, relieving miR-873-5p-mediated repression of TEAD1; TEAD1 transcriptionally activates DERL1 to promote NSCLC malignancy.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); Hippo/YAP (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Luciferase Reporter Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Overexpressed circTTBK2 in NSCLC tissues indicates poor prognosis; circTTBK2 expression is positively associated with TNM stage and lymph node metastasis, implying a potential therapeutic strategy for NSCLC treatment.
Description:

circTTBK2 is a human TTBK2-derived circRNA that is up-regulated in NSCLC tissues and cell lines, and high expression is associated with poorer prognosis, TNM stage and lymph node metastasis. Functionally, circTTBK2 promotes NSCLC cell proliferation, migration, invasion and xenograft tumor growth. Mechanistically, cytoplasmic circTTBK2 sponges miR-873-5p to increase TEAD1, which transcriptionally activates DERL1, forming the circTTBK2/miR-873-5p/TEAD1/DERL1 oncogenic axis.
Confidence score:

0.8655
Other information
Title:

Circular RNA circTTBK2 facilitates non-small-cell lung cancer malignancy through the miR-873-5p/TEAD1/DERL1 axis.

Journal: Epigenomics
Published: 2022
PubMed ID: 35916080
Study type:

combined biological and clinical study
Data availability: www.futuremedicine.com/doi/ suppl/10.2217/epi-2021-0480; circBase (www.circbase.org/); https://starba se.sysu.edu.cn/; http://genome.u csc.edu/; https://jaspar.genereg.net/
Code availability: -