circRNA basic information
circBase ID: -
Name: mmu_circ_Hipk3
Synonym: circHIPK3
Host Gene: Hipk3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005148
MONDO name: type 2 diabetes mellitus
Disease details: type 2 diabetes mellitus
Disease DO ID:
 9352
Disease MeSH ID:
 D003924
Disease NCIt ID:
 C26747
Disease ICD11 ID:
 119724091
Disease OMIM ID:
 125853
Species: Mouse
Species details: Mus musculus
Tissue specimen:

islets / pancreatic islets / mouse islets / rat islets / human islets
Cell lines:

MIN6B1; INS832/13
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: Slc2a2, Akt1, Mtpn
Associated microRNA: miR-124-3p, miR-338-3p, miR-29b-3p, miR-30
Biological function: required to maintain optimal beta-cell functions; promotes glucose-stimulated insulin secretion and insulin biosynthesis; promotes beta-cell proliferation; inhibits beta-cell apoptosis
Molecular mechanism: putative miRNA sponging/sequestration leading to regulation of key beta-cell genes (e.g., Akt1, Slc2a2, Mtpn) and insulin promoter activity
Biological pathway or process:

insulin secretion (promotes); proliferation (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
M
Validation methods:

Microarray; RNA-seq; divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Bioinformatics Analysis; Transfection; Luciferase Reporter Assay; ELISA; IF (Immunofluorescence)
Clinical significance:

-
Description:

circHIPK3 is down-regulated in islets of diabetic db/db mice. Its knockdown in beta-cells impairs glucose-stimulated insulin secretion, reduces beta-cell proliferation, and increases apoptosis, potentially via sequestering multiple miRNAs (e.g., miR-124-3p, miR-338-3p) and regulating key beta-cell genes such as Akt1, Slc2a2, and Mtpn.
Confidence score:

0.7442
Other information
Title:

Circular RNAs as novel regulators of beta-cell functions in normal and disease conditions.

Journal: Molecular metabolism
Published: 2018
PubMed ID: 29396373
Study type:

combined biological and clinical study
Data availability: GSE105096; GSE105097
Code availability: -