circRNA basic information
circBase ID: hsa_circ_0072068
Name: hsa_circ_GOLPH3
Synonym: circGOLPH3
Host Gene: GOLPH3
Genomic location(hg19): chr5:32124823-32126742:-
Genomic location(hg38): chr5:32124717-32126636:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

PC-3; RWPE-1; 293-T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CBX7
Associated microRNA: -
Biological function: Promotes proliferation/cell viability and inhibits apoptosis in prostate cancer cells.
Molecular mechanism: circGOLPH3 binds the protein CBX7 (identified by RNA pull-down/mass spectrometry and validated by CBX7-RIP-qRT-PCR enrichment), and CBX7 phenocopies circGOLPH3 effects on proliferation and apoptosis.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; divergent primers PCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; MTT; EdU Staining; Cell Cycle Assay; TUNEL; Flow Cytometry（Non-apoptosis/cycle）
Clinical significance:

-
Description:

In prostate cancer PC-3 cells, circGOLPH3 is upregulated and promotes proliferation/viability while inhibiting apoptosis. Mechanistically, circGOLPH3 directly binds CBX7, and CBX7 overexpression phenocopies circGOLPH3’s pro-proliferative and anti-apoptotic effects.
Confidence score:

0.6738
Other information
Title:

Regulation of circGOLPH3 and its binding protein CBX7 on the proliferation and apoptosis of prostate cancer cells.

Journal: Bioscience reports
Published: 2020
PubMed ID: 33245100
Study type:

biological research
Data availability: TCGA
Code availability: -