| Expression pattern: |
DN |
| Associated gene: |
p21, PTEN, ITCH |
| Associated microRNA: |
miR-17, miR-224 |
| Biological function: |
Inhibits bladder cancer cell proliferation, migration, invasion and metastasis; induces G1/S cell cycle arrest and apoptosis; suppresses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-17 and miR-224, thereby relieving repression of p21 and PTEN. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
RT-qPCR; Northern Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RNA Pull-Down; FISH / smFISH; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Lower circ-ITCH expression was associated with worse overall survival and circ-ITCH was reported as an independent prognostic marker in BCa. |
| Description: |
circ-ITCH is down-regulated in bladder cancer and functions as a tumor suppressor. It sponges miR-17 and miR-224 to de-repress p21 and PTEN, thereby inhibiting proliferation, migration/invasion, and tumor growth while promoting G1/S arrest and apoptosis. Low circ-ITCH expression is associated with worse overall survival in BCa patients. |
| Confidence score: |
0.802 |