| Expression pattern: |
UP |
| Associated gene: |
ALKBH5, NANOG, Ago2 |
| Associated microRNA: |
miR-1252-5p |
| Biological function: |
promotes temozolomide resistance, proliferation, migration and invasion; inhibits apoptosis; enhances xenograft tumor growth; mediates transfer of resistance via exosomes |
| Molecular mechanism: |
Exosomal circ_0072083 acts as a miRNA sponge for miR-1252-5p, thereby upregulating ALKBH5 and NANOG; it promotes ALKBH5-mediated m6A demethylation of NANOG mRNA to increase NANOG stability; Warburg effect promotes exosomal circ_0072083 release |
| Biological pathway or process: |
ceRNA regulation (promotes); m6A modification (other); glycolysis (promotes); chemoresistance (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; ROC Analysis; Survival Analysis; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circ_0072083 was up-regulated in TMZ-resistant patients, had diagnostic value (AUC=0.85), and high exosomal circ_0072083 level indicated lower overall survival. |
| Description: |
Exosomal circ_0072083 is up-regulated in TMZ-resistant glioma and promotes TMZ chemoresistance. It sponges miR-1252-5p to increase ALKBH5 and NANOG, and enhances ALKBH5-mediated m6A demethylation to stabilize NANOG mRNA. Warburg effect increases the release of exosomal circ_0072083, enabling transfer of resistance to sensitive glioma cells. |
| Confidence score: |
0.8712 |