| Expression pattern: |
UP |
| Associated gene: |
E2F7 |
| Associated microRNA: |
miR-33a-5p |
| Biological function: |
Promotes NSCLC malignant behaviors and attenuates celastrol (Cela)-induced suppression of proliferation, migration and invasion; inhibits apoptosis; promotes cell cycle progression; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-33a-5p to relieve repression of E2F7, thereby attenuating celastrol-mediated anti-tumor effects. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; Luciferase Reporter Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; MTT; Transwell Assay; Western Blot; Bioinformatics Analysis; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ_SATB2 is up-regulated in NSCLC tissues and cell lines. It sponges miR-33a-5p to increase E2F7 expression, thereby promoting malignant phenotypes and weakening celastrol-induced anti-tumor effects, including in xenograft tumors. |
| Confidence score: |
0.7183 |