circRNA basic information
circBase ID: hsa_circ_0001946
Name: hsa_circ_CDR1
Synonym: CDR1as
Host Gene: CDR1
Genomic location(hg19): chrX:139865339-139866824:+
Genomic location(hg38): chrX:140783174-140784659:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005149
MONDO name: pulmonary hypertension
Disease details: pulmonary hypertension
Disease DO ID:
 6432
Disease MeSH ID:
 D006976
Disease NCIt ID:
 -
Disease ICD11 ID:
 1496633964
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HPASMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CAMK2D, CNN3
Associated microRNA: miR-7-5p
Biological function: promotes HPASMC osteogenic differentiation and calcification induced by hypoxia
Molecular mechanism: CDR1as functions as a ceRNA/miRNA sponge for miR-7-5p to upregulate CAMK2D and CNN3
Biological pathway or process:

ceRNA regulation (promotes); proliferation (other); migration (other); invasion (other); metastasis (other); apoptosis (other); cell cycle (other); other pathway/process (promotes)
Detected method:
Q
H
Validation methods:

RT-qPCR; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; IF (Immunofluorescence); Oil Red O Staining; ELISA
Clinical significance:

-
Description:

In hypoxia-induced pulmonary hypertension cellular context, CDR1as is up-regulated in HPASMCs and promotes osteogenic differentiation and calcification. Mechanistically, it sponges miR-7-5p to relieve repression of CAMK2D and CNN3, forming a CDR1as/miR-7-5p/CAMK2D-CNN3 ceRNA axis that drives mineralization.
Confidence score:

0.657
Other information
Title:

circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.

Journal: Molecular therapy. Nucleic acids
Published: 2020
PubMed ID: 33230455
Study type:

biological research
Data availability: -
Code availability: -