| Expression pattern: |
DN |
| Associated gene: |
EIF2B5, JAK2/STAT3 signaling pathway |
| Associated microRNA: |
miR-28 |
| Biological function: |
CircAHNAK suppresses ovarian cancer cell proliferation, migration, invasion, EMT and tumor growth, and promotes apoptosis. |
| Molecular mechanism: |
CircAHNAK acts as a miR-28 sponge to upregulate EIF2B5 expression and inhibit the JAK2/STAT3 signaling pathway. |
| Biological pathway or process: |
JAK/STAT (inhibits); apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; FISH / smFISH; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Manipulating the circAHNAK/miR-28/EIF2B5 axis holds potential to treat ovarian cancers. |
| Description: |
CircAHNAK is down-regulated in ovarian cancer tissues and cell lines and functions as a tumor suppressor. It sponges miR-28 to relieve repression of EIF2B5, thereby inhibiting JAK2/STAT3 signaling, EMT, proliferation, migration, invasion and xenograft tumor growth while promoting apoptosis. |
| Confidence score: |
0.7759 |