| Expression pattern: |
DN |
| Associated gene: |
BRD4, C-myc, EZH2 |
| Associated microRNA: |
miR-27a-3p |
| Biological function: |
Inhibits bladder cancer progression and tumorigenesis by suppressing proliferation/cell-cycle progression; restrains BRD4/C-myc/EZH2 axis. |
| Molecular mechanism: |
circNR3C1 physically binds BRD4, dissociates the BRD4/C-myc promoter complex, inhibits C-myc transcription and downstream EZH2 transcription; additionally reported as miR-27a-3p sponge in prior work. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; FISH / smFISH; IF (Immunofluorescence); Transfection; Western Blot; Cell Cycle Assay; CCK8; In Vivo Animal Model; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
- |
| Description: |
circNR3C1 (from NR3C1) is down-regulated in bladder cancer and suppresses tumor proliferation/tumorigenesis. It binds BRD4 in the nucleus, disrupts the BRD4-C-myc promoter complex, downregulates C-myc and downstream EZH2 transcription, thereby inhibiting BC progression. |
| Confidence score: |
0.7694 |