circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007254
MONDO name: breast cancer
Disease details: Breast cancer
Disease DO ID:
1612
Disease MeSH ID:
-
Disease NCIt ID:
C9335
Disease ICD11 ID:
1047754165
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues; adjacent normal tissues; tumor tissues

Cell lines:

MCF-7; MDA-MB-231; MCF7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: AGR2, HIF-1alpha
Associated microRNA: miR-29a-3p
Biological function: promotes proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: acts as a ceRNA/miRNA sponge for miR-29a-3p to derepress AGR2, thereby increasing HIF-1alpha expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); HIF-1 signaling (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis

Clinical significance:

overexpressed circPVT1 was greatly correlated with positive lymph node examination and tumor size

Description:

circPVT1 is upregulated in breast cancer tissues/cells and promotes proliferation, migration, invasion and tumor growth while inhibiting apoptosis. Mechanistically, it functions as a ceRNA sponge for miR-29a-3p, thereby increasing AGR2 and HIF-1alpha expression via the miR-29a-3p/AGR2/HIF-1alpha axis.

Confidence score:

0.7416

Other information
Title:

CircPVT1 Promoted the Progression of Breast Cancer by Regulating MiR-29a-3p-Mediated AGR2-HIF-1alpha Pathway.

Journal: Cancer management and research
Published: 2020
PubMed ID: 33223849
Study type:

combined biological and clinical study

Data availability: The datasets used and analyzed during the current study are available from the corresponding author on reasonable request, and any data intended for sharing is deidentified.
Code availability: -