| Expression pattern: |
UP |
| Associated gene: |
SLC7A11 |
| Associated microRNA: |
miR-375 |
| Biological function: |
circRPPH1 promotes gastric cancer cell stemness and tumor-initiating ability; circRPPH1 knockdown suppresses stemness marker expression, sphere-formation ability, ALDH activity, and tumor formation ratio. |
| Molecular mechanism: |
circRPPH1 acts as a miR-375 sponge to positively regulate SLC7A11 expression and promotes gastric cancer cell stemness through the miR-375/SLC7A11 axis, likely involving ferroptosis regulation. |
| Biological pathway or process: |
stemness (promotes); ferroptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Transfection; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; CCK8; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
This study identifies circRPPH1 as an up-regulated circRNA in gastric cancer cells and GC cell spheres. circRPPH1 promotes GC cell stemness and tumor-initiating ability by sponging miR-375 and increasing SLC7A11 expression, thereby implicating ferroptosis regulation in the circRPPH1/miR-375/SLC7A11 axis. |
| Confidence score: |
0.6562 |