| Expression pattern: |
UP |
| Associated gene: |
CCNB1 |
| Associated microRNA: |
miR-548b-3p |
| Biological function: |
Oncogenic role in dCCA/CCA: promotes proliferation, migration, and invasion; associated with malignant clinicopathological features. |
| Molecular mechanism: |
ceRNA mechanism: validated binding to miR-548b-3p; proposed downstream target CCNB1 and possible promotion of dCCA progression via miR-548b-3p/CCNB1. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Luciferase Reporter Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Colony Formation Assay |
| Clinical significance: |
Diagnostic biomarker with AUC=0.85; high expression associated with tumor invasion, poor differentiation, and residual tumor. |
| Description: |
hsa_circ_0000673 is up-regulated in distal cholangiocarcinoma and shows diagnostic potential (AUC 0.85). Functionally, its knockdown inhibits CCA cell proliferation, migration, and invasion, supporting an oncogenic role. Mechanistically, it binds miR-548b-3p and is proposed to affect CCNB1 within a ceRNA network. |
| Confidence score: |
0.8162 |