| Expression pattern: |
UP |
| Associated gene: |
TMED2 |
| Associated microRNA: |
miR-186 |
| Biological function: |
Promotes HCC cell growth/proliferation, migration and invasion; inhibits apoptosis; promotes tumour growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: circ_0008305 binds miR-186 to up-regulate TMED2, promoting HCC progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0008305 level was significantly correlated with tumour size, tumour differentiation, TNM stage and metastasis. |
| Description: |
circ_0008305 is up-regulated in HCC tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion and tumour growth). It functions as a ceRNA by sponging miR-186 to relieve repression of TMED2, thereby enhancing HCC progression. |
| Confidence score: |
0.7554 |