| Expression pattern: |
DN |
| Associated gene: |
FMRP, CCAR1, QKI5, beta-catenin |
| Associated microRNA: |
- |
| Biological function: |
Suppresses cancer stemness and malignant behaviors (proliferation, tumorigenicity), and inhibits HCC growth in vivo. |
| Molecular mechanism: |
Acts as an RBP sponge by competitively binding FMRP, thereby blocking FMRP binding to CCAR1 mRNA and deactivating Wnt/beta-catenin signaling; reduced circZKSCAN1 is attributed to downregulated QKI5. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); stemness (inhibits); proliferation (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; RIP (RNA Immunoprecipitation); circRIP; Luciferase Reporter Assay; RNA Pull-Down; IF (Immunofluorescence); Transfection; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; H&E Staining; Survival Analysis; Bioinformatics Analysis; CCK8 |
| Clinical significance: |
Low circZKSCAN1 expression correlates with worse overall and recurrence-free survival; high circZKSCAN1 is a favorable prognostic factor and an independent factor for OS and relapse-free survival. |
| Description: |
circZKSCAN1 is down-regulated in HCC and acts as a tumor suppressor by inhibiting cancer stemness and other malignant phenotypes. Mechanistically it functions as an RBP sponge, competitively binding FMRP to prevent FMRP-CCAR1 mRNA association, thereby reducing CCAR1-driven Wnt/beta-catenin transcriptional activity; its reduction is linked to decreased QKI5. |
| Confidence score: |
0.8826 |