circRNA basic information
circBase ID: hsa_circ_0071474
Name: hsa_circ_NEIL3
Synonym: circNEIL3
Host Gene: NEIL3
Genomic location(hg19): chr4:178274461-178284092:+
Genomic location(hg38): chr4:177353307-177362938:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

cervical cancer tissues; para-carcinoma normal tissues
Cell lines:

End1/E6E7; SiHa; C-4 I; HeLa; C-33A; MDA-MB-231
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KLF12, Ago2
Associated microRNA: miR-137
Biological function: promotes proliferation
Molecular mechanism: circNEIL3 acts as a ceRNA (miRNA sponge) binding miR-137 to indirectly upregulate KLF12 expression.
Biological pathway or process:

proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circNEIL3 (hsa_circ_0071474), derived from NEIL3, is up-regulated in cervical cancer tissues and cell lines. It mainly localizes to the cytoplasm and promotes cervical cancer cell proliferation by sponging miR-137, thereby relieving repression of KLF12 and increasing KLF12 expression; circNEIL3 knockdown suppresses xenograft growth.
Confidence score:

0.8118
Other information
Title:

CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12.

Journal: Cancer cell international
Published: 2021
PubMed ID: 33413360
Study type:

combined biological and clinical study
Data availability: GSE113696; GSE90737
Code availability: -