| Expression pattern: |
DS |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
promotes cell migration and invasion; has little effect on cell proliferation |
| Molecular mechanism: |
- |
| Biological pathway or process: |
migration (promotes); invasion (promotes); proliferation (other) |
| Detected method: |
Q
B
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Clinical Sample Validation |
| Clinical significance: |
F-circEA-2a exists in tumor, but not in the plasma of NSCLC patients with EML4-ALK fusion gene. |
| Description: |
F-circEA-2a is an EML4-ALK-v3b-derived fusion circRNA detected in EML4-ALK-positive NSCLC tumors. It is mainly cytoplasmic and promotes migration and invasion with minimal effect on proliferation, and it was not detectable in patient plasma in this study. |
| Confidence score: |
0.7441 |