| Expression pattern: |
DN |
| Associated gene: |
SIRT3, SOD2, AGO2, ZNF532 mRNA |
| Associated microRNA: |
miR-142-3p |
| Biological function: |
Circ_0,003,423 overexpression protected HUVECs from ox-LDL-induced dysfunction by promoting proliferation, migration and angiogenesis, suppressing apoptosis, and attenuating oxidative injury. |
| Molecular mechanism: |
Circ_0,003,423 acts as a miR-142-3p sponge to positively regulate SIRT3 and activate the SIRT3/SOD2 signaling pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Wound Healing Assay; Tube Formation Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
Restoring circ_0,003,423 expression might be a potential therapeutic strategy for AS patients. |
| Description: |
Circ_0,003,423 was down-regulated in ox-LDL-induced HUVECs used as an in vitro atherosclerosis model. Its overexpression alleviated endothelial cell injury by promoting proliferation, migration and angiogenesis, suppressing apoptosis and oxidative stress, and regulating the miR-142-3p/SIRT3/SOD2 axis. |
| Confidence score: |
0.741 |