| Expression pattern: |
UP |
| Associated gene: |
HMGB1, Ago2 |
| Associated microRNA: |
miR-139-3p |
| Biological function: |
promotes proliferation; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circ_0003645 sponges miR-139-3p to positively regulate HMGB1 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
negatively correlated with prognosis |
| Description: |
circ_0003645 is up-regulated in breast cancer tissues and cell lines and acts oncogenically by promoting proliferation and suppressing apoptosis. Mechanistically, it functions as a ceRNA that sponges miR-139-3p to increase HMGB1 expression, supporting tumor growth in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.7256 |