| Expression pattern: |
UP |
| Associated gene: |
IDH2 |
| Associated microRNA: |
miR-758-3p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, and invasion, inhibits apoptosis, and promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-758-3p to derepress/positively regulate IDH2 expression (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
Circ_0028826 is upregulated in NSCLC tissues and cell lines, mainly localized in the cytoplasm, and promotes proliferation, migration/invasion and tumor growth while inhibiting apoptosis. Mechanistically, it sponges miR-758-3p to derepress IDH2, forming a circ_0028826/miR-758-3p/IDH2 regulatory axis. |
| Confidence score: |
0.7594 |