| Expression pattern: |
UP |
| Associated gene: |
S100A14, Ago2, PTK2 |
| Associated microRNA: |
miR-139-3p |
| Biological function: |
promotes cervical cancer cell proliferation, migration, invasion, angiogenesis, EMT-related malignant behaviors, and tumor growth in vivo |
| Molecular mechanism: |
circ_0003221 acts as a cytoplasmic miRNA sponge for miR-139-3p and positively regulates S100A14 expression through the miR-139-3p/S100A14 regulatory axis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circ_0003221 expression was related to poor 5-year overall survival and circ_0003221 may be a possible diagnostic biomarker for cervical cancer |
| Description: |
circ_0003221 is up-regulated in cervical cancer tissues and cell lines and high expression is associated with poor 5-year overall survival. Functionally, circ_0003221 promotes cervical cancer cell proliferation, migration, invasion, angiogenesis, EMT-related malignant phenotypes, and xenograft tumor growth. Mechanistically, cytoplasmic circ_0003221 sponges miR-139-3p and thereby positively regulates S100A14 expression through the circ_0003221/miR-139-3p/S100A14 axis. |
| Confidence score: |
0.8174 |