| Expression pattern: |
UP |
| Associated gene: |
NOTCH1 |
| Associated microRNA: |
miR-377-3P |
| Biological function: |
promotes RCC cell migration, invasion and metastasis |
| Molecular mechanism: |
acts as a miRNA sponge for miR-377-3P to relieve repression of NOTCH1 |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); EMT (promotes); Notch (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; FISH / smFISH; Transfection; Wound Healing Assay; Transwell Assay; MTT; Luciferase Reporter Assay; Western Blot; RNA-seq; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
High circPDK1 expression is correlated with lymph node metastasis and distant metastasis and may serve as a biomarker for predicting RCC metastasis. |
| Description: |
circPDK1 is up-regulated in RCC tissues and cell lines and promotes RCC cell migration, invasion, and lung metastasis in a xenograft model. Mechanistically, circPDK1 localizes mainly in the cytoplasm and acts as a ceRNA sponge for miR-377-3P, thereby increasing NOTCH1 expression and enhancing metastatic behavior. |
| Confidence score: |
0.7736 |