| Expression pattern: |
UP |
| Associated gene: |
ZEB1 |
| Associated microRNA: |
miR-200b-3p, miR-200c-3p |
| Biological function: |
enhances migration and invasion; promotes invasion and metastasis; promotes mesenchymal phenotype (EMT) |
| Molecular mechanism: |
ceRNA/miRNA sponge: circKDM4C sponges miR-200b-3p and miR-200c-3p to increase ZEB1 expression |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation |
| Clinical significance: |
potential biomarker or therapeutic target for bladder cancer |
| Description: |
circKDM4C is up-regulated in bladder cancer tissues and cell lines and promotes migration, invasion, and metastasis. It primarily localizes to the cytoplasm and acts as a ceRNA by sponging miR-200b-3p/miR-200c-3p, thereby increasing ZEB1 expression and promoting EMT-like mesenchymal features. |
| Confidence score: |
0.8382 |