| Expression pattern: |
UP |
| Associated gene: |
PHGDH, TPST2 |
| Associated microRNA: |
miR-940 |
| Biological function: |
promotes CRC cell proliferation and tumor growth; promotes serine metabolism; increases NADPH and GSH levels and reduces ROS levels under stress conditions |
| Molecular mechanism: |
ceRNA mechanism: circ_0062682 sponges miR-940 to upregulate PHGDH, enhancing serine biosynthesis and supporting tumor growth |
| Biological pathway or process: |
proliferation (promotes); serine metabolism (promotes); oxidative phosphorylation (not specified) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; IF (Immunofluorescence); Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Survival Analysis; ROC Analysis |
| Clinical significance: |
Increased expression of circ_0062682 in CRC notably correlated with a poor prognosis and advanced tumor stage; circ_0062682 expression could differentiate CRC tissues from normal tissues |
| Description: |
In colorectal cancer, circ_0062682 is upregulated and promotes tumor growth by enhancing serine biosynthesis. It mainly functions in the cytoplasm as a ceRNA that sponges miR-940, thereby releasing PHGDH expression and increasing serine-related metabolic outputs (e.g., NADPH/GSH) that support proliferation and survival under stress. |
| Confidence score: |
0.8673 |