| Expression pattern: |
UP |
| Associated gene: |
FOXM1 |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
Circ_0025033 promotes paclitaxel resistance and malignant behaviors of paclitaxel-resistant ovarian cancer cells, including migration and invasion, and inhibits apoptosis; circ_0025033 knockdown enhances PTX sensitivity, suppresses migration/invasion, and induces apoptosis. |
| Molecular mechanism: |
circ_0025033 functions through a ceRNA mechanism by sponging miR-532-3p to upregulate FOXM1 expression; circ_0025033-deficient exosomes also repress malignant activities of PTX-resistant OC cells. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
circ_0025033 may serve as a biomarker in the development of ovarian cancer chemoresistance. |
| Description: |
circ_0025033 is up-regulated in ovarian cancer tissues and cells, with higher expression in paclitaxel-resistant ovarian cancer cells. Functionally, circ_0025033 promotes PTX resistance, migration, and invasion while suppressing apoptosis, and its knockdown reverses these malignant phenotypes. Mechanistically, circ_0025033 acts as a ceRNA for miR-532-3p to upregulate FOXM1, and circ_0025033-deficient exosomes can also inhibit PTX resistance and malignant behavior. |
| Confidence score: |
0.7795 |