| Expression pattern: |
UP |
| Associated gene: |
FRAS1, DGCR8 |
| Associated microRNA: |
miR-761, miR-192-3p |
| Biological function: |
Enhances adhesion, migration and invasion of CRC cells and promotes colorectal liver metastasis; associated with worse overall survival. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-761 and miR-192-3p to up-regulate FRAS1; binds DGCR8 and alters its nuclear/cytosolic distribution, indirectly reducing mature miR-761 and miR-192-3p in cytoplasm. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (other); TGF-beta/SMAD (other); Notch (other); Wnt/beta-catenin (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis; Survival Analysis; Cohort Study; Clinical Sample Validation |
| Clinical significance: |
High circ102049 levels correlate with worse 5-year overall survival and act as an independent prognostic factor in CRC; associated with liver metastasis. |
| Description: |
circ102049 is up-regulated in CRC with liver metastasis and predicts poor survival. It promotes CRC cell adhesion/migration/invasion and liver metastasis mainly by sponging miR-761 and miR-192-3p to increase FRAS1, and it also binds DGCR8 to alter its localization, indirectly affecting miR-761/miR-192-3p maturation. |
| Confidence score: |
0.887 |