| Expression pattern: |
UP |
| Associated gene: |
DVL3 |
| Associated microRNA: |
miR-665 |
| Biological function: |
Circ_0101802 promotes CRC cell proliferation, migration, angiogenesis/tube formation and xenograft tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
ceRNA mechanism: circ_0101802 sponges miR-665 to positively regulate DVL3 protein expression in CRC cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); angiogenesis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0101802 expression was associated with short survival time, low survival rate, poorly histological grade, advanced TNM stage, and positive lymph node metastasis in CRC patients; circ_0101802 might be a novel prognostic marker. |
| Description: |
Circ_0101802 is up-regulated in colorectal cancer tissues and cell lines and high expression predicts poor survival. Functionally, circ_0101802 promotes CRC proliferation, migration, angiogenesis/tube formation and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-665 to increase DVL3 protein expression. |
| Confidence score: |
0.8466 |