circRNA basic information
circBase ID: hsa_circ_0000467
Name: hsa_circ_SKA3
Synonym: circ_0000467
Host Gene: SKA3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue; paracancerous tissue

Cell lines:

HT29; HCT8; SW620; DLD-1; NCM460; HEK-293T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3B
Associated microRNA: miR-651-5p
Biological function: Promotes CRC cell growth, migration, invasion and metastatic potential; knockdown represses these malignant characteristics.
Molecular mechanism: circ_0000467 acts as a miRNA sponge for miR-651-5p and up-regulates DNMT3B expression through the miR-651-5p/DNMT3B axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; BrdU; Transwell Assay; RIP (RNA Immunoprecipitation); Western Blot; Luciferase Reporter Assay

Clinical significance:

Circ_0000467 may be a potential diagnostic biomarker and therapeutic target for CRC.

Description:

circ_0000467 is up-regulated in colorectal cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes CRC cell growth, migration and invasion by sponging miR-651-5p, thereby increasing DNMT3B expression, and may serve as a potential diagnostic biomarker and therapeutic target.

Confidence score:

0.7581

Other information
Title:

Circ_0000467 modulates malignant characteristics of colorectal cancer via sponging miR-651-5p and up-regulating DNMT3B.

Journal: Nucleosides, nucleotides & nucleic acids
Published: 2023
PubMed ID: 36067529
Study type:

combined biological and clinical study

Data availability: GSE142837; GSE138589; GEO database (https:// www.ncbi.nlm.nih.gov/geo/); data available from the corresponding author upon request
Code availability: -