| Expression pattern: |
UP |
| Associated gene: |
AKT1, mTOR, QKI |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
promotes proliferation, migration and invasion; promotes EMT and metastasis; promotes glycolysis/Warburg effect and inhibits autophagy in gastric cancer |
| Molecular mechanism: |
acts as a miRNA sponge (ceRNA) for miR-149-5p to regulate AKT1 and activate AKT1/mTOR signaling; circNRIP1 is transmitted via exosomes; QKI promotes circNRIP1 biogenesis |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); glycolysis (promotes); autophagy (inhibits); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); Northern Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
Patients who had high levels of circNRIP1 within their GC tissues had significantly shorter overall survival and disease-free survival; circNRIP1 expression correlated with tumour size and lymphatic invasion; circNRIP1 was higher in plasmatic exosomes from GC patients than normal subjects. |
| Description: |
circNRIP1 is upregulated in gastric cancer and promotes malignant phenotypes by sponging miR-149-5p, thereby increasing AKT1 and activating the AKT/mTOR axis to enhance glycolysis/Warburg effect, EMT, and metastasis while suppressing autophagy. circNRIP1 can be transferred via exosomes and its biogenesis is promoted by the RBP QKI; high circNRIP1 associates with worse survival. |
| Confidence score: |
0.8921 |