circRNA basic information
circBase ID: hsa_circ_0072995
Name: hsa_circ_RGNEF
Synonym: Circ_0072995
Host Gene: RGNEF
Genomic location(hg19): chr5:73069679-73076570:+
Genomic location(hg38): chr5:73773854-73780745:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Tumor tissues; matched non-tumor tissue (normal) samples
Cell lines:

SKBR3; HCC38; MCF-7; MDA-MB-231; BT-549; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SHMT2
Associated microRNA: miR-149-5p
Biological function: Promotes malignant phenotypes (proliferation, migration, invasion, adhesion), inhibits apoptosis, and promotes anaerobic glycolysis/tumor growth in breast cancer.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-149-5p to up-regulate SHMT2; contributes to glycolysis-associated malignant behaviors.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; divergent primers PCR; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

Promising molecular target for breast cancer treatment.
Description:

Circ_0072995 is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It sponges miR-149-5p to increase SHMT2 expression, promoting malignant phenotypes and anaerobic glycolysis, and its silencing suppresses xenograft tumor growth.
Confidence score:

0.7591
Other information
Title:

Circ_0072995 Promotes Cell Carcinogenesis via Up-Regulating miR-149-5p-Mediated SHMT2 in Breast Cancer.

Journal: Cancer management and research
Published: 2020
PubMed ID: 33173349
Study type:

combined biological and clinical study
Data availability: starBase (RNA-seq data of 1104 breast cancer and 113 adjacent non-tumor tissues)
Code availability: -