| Expression pattern: |
UP |
| Associated gene: |
HDAC4 |
| Associated microRNA: |
miR-432-5p |
| Biological function: |
Promotes osteosarcoma cell proliferation, migration and invasion, and inhibits autophagy; circCRIM1 knockdown suppresses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-432-5p to up-regulate HDAC4 (ceRNA axis), thereby promoting osteosarcoma progression and suppressing autophagy. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); autophagy (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; MTT; Transwell Assay; Western Blot; Luciferase Reporter Assay; Transfection; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
circCRIM1 expression was associated with tumor size and differentiation grade; proposed as a diagnostic/therapeutic target for OS. |
| Description: |
circCRIM1 is up-regulated in osteosarcoma tissues and cell lines. It promotes proliferation, migration and invasion and suppresses autophagy by sponging miR-432-5p, which leads to increased HDAC4 expression and enhanced tumor growth in vivo. |
| Confidence score: |
0.6952 |