| Expression pattern: |
UP |
| Associated gene: |
ENO1, EIF4A3, SEMA5A, Akt, beta-catenin |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Promotes proliferation, migration, invasion, angiogenesis and glycolysis, and suppresses apoptosis in bladder cancer; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-330-5p to upregulate ENO1 and activate Akt and beta-catenin signaling; recruits EIF4A3 to enhance SEMA5A mRNA stability and increase SEMA5A expression, promoting angiogenesis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); angiogenesis (promotes); glycolysis (promotes); PI3K/AKT (promotes); Wnt/beta-catenin (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circSEMA5A expression was associated with tumor stage and tumor size; suggested as a potential biomarker and therapeutic target for BC. |
| Description: |
circSEMA5A is upregulated in bladder cancer and promotes malignant phenotypes (proliferation, migration/invasion, angiogenesis) while inhibiting apoptosis and enhancing glycolysis. It functions via a ceRNA axis circSEMA5A/miR-330-5p/ENO1 that activates Akt and beta-catenin signaling, and also recruits EIF4A3 to stabilize SEMA5A mRNA to promote angiogenesis. |
| Confidence score: |
0.8279 |