| Expression pattern: |
DN |
| Associated gene: |
AGO2, KDM4A, YAP1 |
| Associated microRNA: |
miR-526b |
| Biological function: |
inhibits gastric cancer cell proliferation and invasion, promotes apoptosis, and acts as an anti-oncogene |
| Molecular mechanism: |
circ_SPECC1 adsorbs miR-526b and enhances the inhibitory effect of miR-526b on the downstream KDM4A/YAP1 pathway |
| Biological pathway or process: |
Hippo/YAP (inhibits); proliferation (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
may provide new targets for the prevention and treatment of gastric cancer |
| Description: |
circ_SPECC1 is down-regulated in gastric cancer cell lines and functions as a tumor suppressor by inhibiting proliferation and invasion while promoting apoptosis. Mechanistically, it binds miR-526b and enhances miR-526b-mediated inhibition of the KDM4A/YAP1 pathway, suggesting potential therapeutic relevance in gastric cancer. |
| Confidence score: |
0.6853 |