| Expression pattern: |
DN |
| Associated gene: |
PCDH10, AGO2 |
| Associated microRNA: |
miR-576-5p |
| Biological function: |
Suppresses CRC progression by inhibiting proliferation, migration/invasion, metastasis, EMT and stemness, and promoting apoptosis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-576-5p to relieve repression of PCDH10, thereby inhibiting Wnt/beta-catenin signaling and EMT/stemness. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); stemness (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Western Blot; Survival Analysis |
| Clinical significance: |
Higher hsa_circ_0001666 expression indicates better overall survival and is associated with lower TNM stage, less lymph node invasion and smaller tumour size. |
| Description: |
In colorectal cancer, hsa_circ_0001666 is down-regulated and functions as a tumor suppressor. It sponges miR-576-5p to de-repress PCDH10, thereby inhibiting Wnt/beta-catenin signaling, EMT/stemness, proliferation and metastasis while promoting apoptosis. Higher expression is associated with better patient survival. |
| Confidence score: |
0.8748 |