circRNA basic information
circBase ID: hsa_circ_0001421
Name: hsa_circ_SEC31A
Synonym: circ_0001421
Host Gene: SEC31A
Genomic location(hg19): chr4:83799882-83803090:-
Genomic location(hg38): chr4:82878729-82881937:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: Lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung cancer tissues; adjacent normal tissues; xenograft tumors
Cell lines:

A549; H1299; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CDCA3, HK2
Associated microRNA: miR-4677-3p
Biological function: Promotes lung cancer cell proliferation, migration, invasion, glycolysis, and tumor growth; circ_0001421 knockdown suppresses these phenotypes.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-4677-3p, relieving repression of CDCA3; regulates glycolysis-associated HK2 expression.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; MTT; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model
Clinical significance:

High Hsa_circ_0001421 expression is associated with advanced TNM stages, larger tumor size, and deeper invasion depth in lung cancer.
Description:

circ_0001421 is up-regulated in lung cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, glycolysis, and in vivo tumor growth by sponging miR-4677-3p to upregulate CDCA3, with associated increases in HK2 and glycolytic output.
Confidence score:

0.6261
Other information
Title:

Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3.

Journal: Diagnostic pathology
Published: 2020
PubMed ID: 33109222
Study type:

combined biological and clinical study
Data availability: The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -