| Expression pattern: |
DS |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Does not affect AR signaling, prostate cancer cell proliferation, or invasion. |
| Molecular mechanism: |
Not specified; circAR3 did not regulate AR transcriptional activity or downstream AR target gene expression in tested conditions. |
| Biological pathway or process: |
proliferation (other); invasion (other) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; Luciferase Reporter Assay; Western Blot; MTT; Transwell Assay; FISH / smFISH; Clinical Sample Validation; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Plasma circAR3 levels are positively associated with high Gleason scores and lymph node metastasis, and become undetectable after radical prostatectomy; proposed as a biomarker to monitor PCa development and progression. |
| Description: |
This study characterizes circAR3 (from the AR gene) as a prostate/PCa-derived circRNA that is detectable in patient plasma and mouse xenograft serum. Tissue circAR3 decreases with progression to CRPC, while plasma circAR3 is higher in high-risk primary PCa (high Gleason score, lymph node metastasis) and disappears after prostatectomy. Functionally, circAR3 does not regulate AR signaling, proliferation, or invasion in tested PCa cell models. |
| Confidence score: |
0.8043 |